Technical introduction
High purity activated NK (H-NK) technology uses feeder cell lines to stably express IL-12, IL-15, IL-18, IL-21 and other cytokines and co-stimulatory molecules (4-1BBL ), so that the above cytokines are expressed on the cell membrane and become transmembrane proteins. Feeder cells were collected by irradiation and centrifugation, and co-cultured with PBMC to selectively expand NK cells.
The number of NK cells obtained by this method was expanded by 141.67±26.13 times, the killing function (CD107a degranulation) was increased by about 4 times, and the function of secreting cytokines (intracellular IF Ng expression) was increased by about 15 times.
Technical principle
The main effector cells of NK cell therapy are CD56+/CD16+/CD3- lymphocytes. Plays a key role in fighting tumors and viral infections. Under normal circumstances, the immune surveillance functions of NK cells and CTL cells complement and coordinate with each other to jointly inhibit the occurrence and development of tumor cells. Its main killing methods can be divided into the following three ways:
1. NK cells can directly kill tumor cells NK cells can recognize tumor cells through various mechanisms, release toxic particles such as granzyme and perforin, and cause the lysis of tumor cells.
2. A large number of inflammatory cytokines released by NK cells have the effect of inhibiting and killing tumors. NK cells cultured in vitro can secrete a variety of cytokines, such as IFN-1, TNF-1, IL-2, etc., which not only have a direct inhibitory effect on tumor cells, but also It can also indirectly kill tumor cells by regulating the reactivity of the body's immune system.
3. NK cells can induce apoptosis of tumor cells. NK cells express Fas1 (type II transmembrane glycoprotein) during the culture process and induce tumor cell apoptosis by combining with Fas (type I transmembrane glycoprotein) expressed on the tumor cell membrane.
H-NK technical advantages (compared with conventional NK)
Advantages of clinical application of H-NK cell therapy technology
1. Recovery of immune damage caused by surgery;
2. Specific removal of residual tumor cells after operation to prevent metastasis and recurrence;
3. Enhance the sensitivity of radiotherapy and reduce the toxic and side effects of radiotherapy;
4. Resist the immunosuppressive effect of chemotherapeutic drugs;
5. Enhance the sensitivity to chemotherapy drugs;
6. Support immune reconstruction after bone marrow failure;